17th International Fiber and Polymer Research Symposium

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Bioactive compound-loaded PVA nanofibers inhibit the proliferation of Glioblastoma cells

Authors :

Melis Ercelik¹ Cagla Tekin² Busra Mutlu³ Fatmanur Parin⁴ Kenan Yildirim⁵ Berrin Tunca⁶

1- Bursa Uludag University 2- Bursa Uludag University 3- Bursa Technical University 4- Bursa Technical University 5- Bursa Technical University 6- Bursa Uludag University

Keywords :

Glioblastoma،PVA nanofiber،Olea europaea leaf extract phenolics

Abstract :

Glioblastoma (GB) is the most aggressive brain tumor and has a median survival of only 15 months despite the current treatment protocol. One of the most significant factors in the failure of GB treatment is the inadequacy of Temozolomide (TMZ), which is used as chemotherapy, and the development of recurrence in a short time. For this reason, treatment approaches that can be used locally are needed to prevent relapse. This study aimed to characterize the encapsulation of oleuropein (OL), hydroxytyrosol (HT), tyrosol (TYR), and rutin from TMZ and Olea europaea leaf extract (OLE) phenolics into polyvinyl alcohol (PVA) nanofibers using electrospinning and investigate their effect on T98G cell proliferation. Release studies of molecules from PVA nanofibers in PBS were evaluated by UV/VIS spectrophotometry, and their effects on T98G cell growth were assessed by real-time cell tracking analysis. The morphological properties of PVA nanofibers loaded with compounds were determined by SEM. The loaded nanofibers were burst-released in PBS and UV calibration curves were created for molecules. No cytotoxic effect of unloaded empty PVA was observed on T98G cells. Each PVA nanofiber loaded with molecules significantly suppressed T98G cell viability (p<0.0001). OL and rutin are the most suitable due to their compatibility with PVA and their effects on T98G and L929 cell proliferation. The fiber diameters of PVA-TMZ, PVA-OL, PVA-rutin nanofibers increased with increasing solution viscosity. Thus, our data showed that loaded PVAs could be an effective candidate therapeutic tool that could inhibit the proliferation of GB cells.